Regulation of T Cell Trafficking by Enzymatic Synthesis of O-Glycans

Regulation of T Cell Trafficking by Enzymatic Synthesis of O-Glycans

Blog Article

Selectins constitute a family of oligosaccharide binding proteins that play critical roles in regulating the trafficking of leukocytes.In T cells, L-selectin (CD62L) controls the here capacity for naive and memory T cells to actively survey peripheral lymph nodes, whereas P- and E-selectin capture activated T cells on inflamed vascular endothelium to initiate extravasation into non-lymphoid tissues.The capacity for T cells to interact with all of these selectins is dependent on the enzymatic synthesis of complex O-glycans, and thus, this protein modification plays an indispensable role in regulating the distribution and homing of both naive and previously activated T cells in vivo.In contrast to neutrophils, O-glycan synthesis is highly dynamic in T cell populations and is largely controlled by extracellular stimuli such as antigen recognition or signaling though cytokine receptors.

Herein, we review the basic principles of enzymatic synthesis of complex O-glycans, discuss tools and reagents for studying this type bostik roll-cote of protein modification and highlight our current understanding of how O-glycan synthesis is regulated and subsequently impacts the trafficking potential of diverse T cell populations.